Main Article Content
Abstract
Penelitian bertujuan untuk mengetahui hasil pemisahan sperma X dan Y pada semen domba dari berbagai panjang elektroda. Materi yang digunakan yaitu 6 ekor domba jantan yang mempunyai semen dengan minimal volume 0,4 ml dan motilitas 70%. Penelitian menggunakan rancangan percobaan bujur sangkar latin dengan perlakuan panjang elektroda 1,0; 3,5 dan 7,0 cm. Penampungan semen dilakukan menggunakan vagina buatan setiap minggu sekali dan sebanyak 3 kali per ekor selama penelitian. Setiap semen hasil penampungan diencerkan, kemudian dilakukan pemisahan antara sperma X dan Y. Parameter yang diamati adalah motilitas, daya hidup, abnormalitas, morfometri sperma. Data dianalisis menggunakan statistik diskriptif, uji t’student, regresi dan korelasi. Hasil penelitian menunjukkan bahwa kualitas sperma yang terbaik dan terburuk masing-masing dihasilkan panjang elektroda 1 cm dan 7 cm, kualitas sperma mempunyai hubungan yang nyata (P<0,05) dan keeratan hubungan yang nyata (P<0,05) terhadap panjang elektroda. Sedangkan hasil pemisahan sperma X dan Y tidak berbeda nyata pada ketiga perlakuan, dan panjang elektroda tidak menunjukkan ada hubungan yang nyata dengan hasil pemisahan sperma. Kesimpulan, panjang elektroda tidak berpengaruh terhadap persentase sperma X dan Y dari hasil pemisahan.
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References
- Carvalho, J. O., R. Sartori and M. A. N. Dodec. 2014. Different ways to evaluate bovine sexed sperm in vitro. Anim. Reprod. 11 (3): 199-206.
- Carvalhoa, J. O., R. Sartoria, L. Rodellob, G. B. Mourãoa, S. D. Bicudob and M. A. N. Dodec. 2018. Flow cytometry sex sorting affects bull sperm longevity and compromises their capacity to bind to oviductal cells. Livestock Sci. 207: 30-37.
- Garner, D. L. and E. S. E. Hafez. 1993. Spermatozoa and Seminal Plasma. In : E.S.E. Hafez (editor) Reproduction in Farm Animals. 6th Ed. Lea & Febiger, Philadelphia.
- Garner, D. L and G. E. Seidel, Jr. 2000. Sexing Bull Sperm. In Tropics in Bull Fertility. Chenoweth, P. J (Ed). International Veterinary Information service Forf Collins, Colorodo.
- Hafez, E. S. E. 1993. X and Y Chromosome – Bearing Spermatozoa. In : E. S. E. Hafez (editor) Reproduction in Farm Animals. 6th Ed. Lea & Febiger, Philadelphia.
- Hough, S. R. and R. H. Foote. 2002. Effect of density gradient osmolality on specific gravity of bull sperm and their separation, but not on basis of sex. J. Reprod and Develop. 48: 399-407.
- Kemeterian Pertanian 2015. Produksi Daging Domba Menurut Provinsi. Donwload www.pertanian.go.id/ap_pages/mod/datanak. diakses 10 Maret 2017.
- Malik, A., A. W. Haron, R. Yusoff, M. Bukar, A. Kasim and M. Sabri. 2011. Verification of X and Y spermatozoa separation by nested polymerase chain reaction (PCR), Motility and membrane integrity in bovine. Afric. J. Biotec. 10 (85): 19796-19801.
- Monteiro, P. L. J. Jr., A. M. Batista, F. C. Almeida, A. E. S. Figueiredo, P. C. Soares, G. F. Carneiro and M. M. P. Guerra. 2016. Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen. Anim. Reprod., Belo Horizonte. 13 (2): 112-116.
- Moore, K. and W. W. Thatcher. 2006. Major Advances Associated with Reproduction in Dairy Cattle. Department of Animal Sciences, University of Florida. J. Dairy Science. 89: 1254-1266.
- Partodihardjo, S. 1987. Ilmu Reproduksi Hewan. Cetakan ke 2 Penerbit Mutiara Sumber Swadaya. Jakarta.
- Pindaru, L., I. M. Balaci and I. S. Groza. 2016. Sperm sexing technology - new directions in medicine Tehnologia sex?rii materialului seminal - o nou? direc?ie în medicin?. Revista Român? de Medicin? de Laborator. 24 (1): 111-121.
- Rath, D., S. Barcikowski, S. de Graaf, W. Garrels, R. Grossfeld, S. Klein, W. Knabe, C’ Knorr, W. Kues, H, Meyer, J. Michl, G. Moench-Tegeder, C. Rehbock, U. Taylor and S. Washausen. 2013. Sex selection of sperm in farm animals: status report and developmental prospects. Reprod. 145: 15-30
- Sharma, M. and N. Sharma. 2016. Sperm Sexing in Animals; Review Article. Adv Anim. Vet. Sci. 4 (10): 543-549.
- Steel, R. G. D. dan J. H. Torrie. 1989. Prinsip dan Prosedur Satistika. Edisi Kedua. Alih Bahasa Bambang Sumantri. Penerbit: PT Gramedia, Jakarta.
- Toelihere, M. R. 1985. Inseminasi Buatan pada Ternak. Penerbit Angkasa, Bandung.
- Trigal, B., E. Gómeza, J. N. Caamañoa, M. Muñoza, J. Morenob, S. Carroceraa, D. Martína and C. Dieza. 2012. In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm. Theriogenology 78: 1465–1475.
- Tubman, L. M., Z. Brink, T. K. Suk and G. E. Seidel.Jr. 2004. Characteristics of Calves produced with sperm sexed by flow cytometry/cel sorting. J. Anim. Sci. 82: 1029–1036.
- Vazquez, F. A. G., J. Gadeal, C. Matas and W. V. Holt. 2016. Importance of sperm morphology during sperm transport and fertilization in mammals. Asian J. Andrology 18: 1–7.
References
Carvalho, J. O., R. Sartori and M. A. N. Dodec. 2014. Different ways to evaluate bovine sexed sperm in vitro. Anim. Reprod. 11 (3): 199-206.
Carvalhoa, J. O., R. Sartoria, L. Rodellob, G. B. Mourãoa, S. D. Bicudob and M. A. N. Dodec. 2018. Flow cytometry sex sorting affects bull sperm longevity and compromises their capacity to bind to oviductal cells. Livestock Sci. 207: 30-37.
Garner, D. L. and E. S. E. Hafez. 1993. Spermatozoa and Seminal Plasma. In : E.S.E. Hafez (editor) Reproduction in Farm Animals. 6th Ed. Lea & Febiger, Philadelphia.
Garner, D. L and G. E. Seidel, Jr. 2000. Sexing Bull Sperm. In Tropics in Bull Fertility. Chenoweth, P. J (Ed). International Veterinary Information service Forf Collins, Colorodo.
Hafez, E. S. E. 1993. X and Y Chromosome – Bearing Spermatozoa. In : E. S. E. Hafez (editor) Reproduction in Farm Animals. 6th Ed. Lea & Febiger, Philadelphia.
Hough, S. R. and R. H. Foote. 2002. Effect of density gradient osmolality on specific gravity of bull sperm and their separation, but not on basis of sex. J. Reprod and Develop. 48: 399-407.
Kemeterian Pertanian 2015. Produksi Daging Domba Menurut Provinsi. Donwload www.pertanian.go.id/ap_pages/mod/datanak. diakses 10 Maret 2017.
Malik, A., A. W. Haron, R. Yusoff, M. Bukar, A. Kasim and M. Sabri. 2011. Verification of X and Y spermatozoa separation by nested polymerase chain reaction (PCR), Motility and membrane integrity in bovine. Afric. J. Biotec. 10 (85): 19796-19801.
Monteiro, P. L. J. Jr., A. M. Batista, F. C. Almeida, A. E. S. Figueiredo, P. C. Soares, G. F. Carneiro and M. M. P. Guerra. 2016. Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen. Anim. Reprod., Belo Horizonte. 13 (2): 112-116.
Moore, K. and W. W. Thatcher. 2006. Major Advances Associated with Reproduction in Dairy Cattle. Department of Animal Sciences, University of Florida. J. Dairy Science. 89: 1254-1266.
Partodihardjo, S. 1987. Ilmu Reproduksi Hewan. Cetakan ke 2 Penerbit Mutiara Sumber Swadaya. Jakarta.
Pindaru, L., I. M. Balaci and I. S. Groza. 2016. Sperm sexing technology - new directions in medicine Tehnologia sex?rii materialului seminal - o nou? direc?ie în medicin?. Revista Român? de Medicin? de Laborator. 24 (1): 111-121.
Rath, D., S. Barcikowski, S. de Graaf, W. Garrels, R. Grossfeld, S. Klein, W. Knabe, C’ Knorr, W. Kues, H, Meyer, J. Michl, G. Moench-Tegeder, C. Rehbock, U. Taylor and S. Washausen. 2013. Sex selection of sperm in farm animals: status report and developmental prospects. Reprod. 145: 15-30
Sharma, M. and N. Sharma. 2016. Sperm Sexing in Animals; Review Article. Adv Anim. Vet. Sci. 4 (10): 543-549.
Steel, R. G. D. dan J. H. Torrie. 1989. Prinsip dan Prosedur Satistika. Edisi Kedua. Alih Bahasa Bambang Sumantri. Penerbit: PT Gramedia, Jakarta.
Toelihere, M. R. 1985. Inseminasi Buatan pada Ternak. Penerbit Angkasa, Bandung.
Trigal, B., E. Gómeza, J. N. Caamañoa, M. Muñoza, J. Morenob, S. Carroceraa, D. Martína and C. Dieza. 2012. In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm. Theriogenology 78: 1465–1475.
Tubman, L. M., Z. Brink, T. K. Suk and G. E. Seidel.Jr. 2004. Characteristics of Calves produced with sperm sexed by flow cytometry/cel sorting. J. Anim. Sci. 82: 1029–1036.
Vazquez, F. A. G., J. Gadeal, C. Matas and W. V. Holt. 2016. Importance of sperm morphology during sperm transport and fertilization in mammals. Asian J. Andrology 18: 1–7.