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Abstract
This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.
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References
- Arlina, F., Husmaini, S., Rhoudha, R., Sardi, W.R., Rafian, T., 2022. Keragaman Fenotipe Kualitatif dan Kuantitatif Itik Sikumbang Jonti sebagai Plasma Nutfah di Sumatera Barat. J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.) 11, 291–299. https://doi.org/10.46549/jipvet.v11i3.173
- Ghanem, H.M., Ateya, A.I., Saleh, R.M., Saleh, M., 2017. Artificial Insemination vs Natural Mating and Genetic PRL/PstI Locus Polymorphism and Their Effect on Different Productive and Reproductive Aspects in Duck. Advances in Animal and Veterinary Sciences 5, 179–184. https://doi.org/10.17582/journal.aavs/2017/5.4.179.184
- Indriati, M., Sumantri, C., Susanti, T., 2015. Analysis of Prolactin Gene Exon 4 Diversity in Peking, White Mojosari, and Peking White Mojosari Crossbreed. Med Pet 39, 14–19. https://doi.org/10.5398/medpet.2016.39.1.14
- Kansaku, N., Ohkubo, T., Okabayashi, H., Guémené, D., Kuhnlein, U., Zadworny, D., Shimada, K., 2005. Cloning of duck PRL cDNA and genomic DNA. General and Comparative Endocrinology 141, 39–47. https://doi.org/10.1016/j.ygcen.2004. 11.017
- Masti, H., Yurnalis, Y., Nova, T.D., Kamsa, Z., Rafian, T., 2021. Keragaman Gen Growth Hormone Receptor (GHR) Ekson 10 pada itik Sikumbang Janti. JIPTHP 9, 15–19. https://doi.org/10.29244/jipthp.9.1.15-19
- Noor, R.R., 2010. Genetika Ternak, 6th ed. Penebar Swadaya, Jakarta.
- Nova, T.D., Anggraeni, S.D., Wardiansyah, M., Ramadhani, E.P., 2019. Frekuensi Pemberian Ransum Secara Periodik dan Level Protein Terhadap Karkas dan Gambaran Darah Itik Lokal Sikumbang Janti. JPI 21, 64. https://doi.org/10.25077/jpi.21.2.64-75.2019
- Nova, T.D., Syahruddin, E., Zein, R., 2020. The Productivity of Duck in Different Temperature Cage Management. J.Nat.Indonesia 18, 43. https://doi.org/10.31258/jnat.18.1.43-61
- Purwantini, D., Santosa, R.S.S., Santosa, S.A., Susanto, A., Candrasari, D.P., Ismoyowati, I., 2020. Prolactin gene polymorphisms and associations with reproductive traits in Indonesian local ducks. Vet World 13, 2301–2311. https://doi.org/10.14202/vetworld.2020.2301-2311
- Saputro, B.E., Sutrisna, R., Santosa, P.E., Fathul, F., 2016. Pengaruh Ransum yang Berbeda pada Itik Jantan Terhadap Jumlah Leukosit dan Diferensial Leukosit. Jurnal Ilmiah Peternakan Terpadu 4, 176–181.
- Setyo Budi, E., Yektiningsih, E., Priyanto, E., 2015. Profitabilitas Usaha Ternak Itik Petelur di Desa Kebonsari Kecamatan Candi, Sidoarjo. AGR 1, 32–37. https://doi.org/10.18196/agr.115
- Subekti, K., Solihin, D.D., Afnan, R., Gunawan, A., Sumantri, C., 2019. Polymorphism of Duck HSP70 Gene and mRNA Expression under Heat Stress Conditions. International J. of Poultry Science 18, 591–597. https://doi.org/10.3923/ijps.2019.591.597
- Susanti, T., 2015. Prolactin as a Candidate Gene Controlling Molting and Egg Production of Duck. Indonesian Bulletin of Animal and Veterinary Sciences 25, 23–28. https://doi.org/10.14334/wartazoa.v25i1.1125
- Susanti, T., Noor, R.R., Hardjosworo, P.S., Prasetyo, L.H., 2012. Relationship Between Prolactin Hormone Level, Molting and Duck Egg Production. J. Indonesian Trop. Anim. Agric. 37, 161–167. https://doi.org/10.14710/jitaa.37.3.161-167
- Wang, C., Liang, Z., Liang, Z., Yu, W., Yu, W., Feng, Y., Feng, Y., Peng, X., Peng, X., Gong, Y., Gong, Y., Li, S., Li, S., 2011. Polymorphism of the prolactin gene and its association with egg production traits in native Chinese ducks. SA J. An. Sci. 41, 63–69. https://doi.org/10.4314/sajas.v41i1.66044
- Yurnalis, Y., Husmaini, H., Sabrina, S., 2017. Polymorphisms of Growth Hormone Gene Exon 1 and their Associations with Body Weight in Pitalah and Kumbang Janti Ducks. International J. of Poultry Science 16, 203–208. https://doi.org/10.3923/ijps.2017.203.208
References
Arlina, F., Husmaini, S., Rhoudha, R., Sardi, W.R., Rafian, T., 2022. Keragaman Fenotipe Kualitatif dan Kuantitatif Itik Sikumbang Jonti sebagai Plasma Nutfah di Sumatera Barat. J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.) 11, 291–299. https://doi.org/10.46549/jipvet.v11i3.173
Ghanem, H.M., Ateya, A.I., Saleh, R.M., Saleh, M., 2017. Artificial Insemination vs Natural Mating and Genetic PRL/PstI Locus Polymorphism and Their Effect on Different Productive and Reproductive Aspects in Duck. Advances in Animal and Veterinary Sciences 5, 179–184. https://doi.org/10.17582/journal.aavs/2017/5.4.179.184
Indriati, M., Sumantri, C., Susanti, T., 2015. Analysis of Prolactin Gene Exon 4 Diversity in Peking, White Mojosari, and Peking White Mojosari Crossbreed. Med Pet 39, 14–19. https://doi.org/10.5398/medpet.2016.39.1.14
Kansaku, N., Ohkubo, T., Okabayashi, H., Guémené, D., Kuhnlein, U., Zadworny, D., Shimada, K., 2005. Cloning of duck PRL cDNA and genomic DNA. General and Comparative Endocrinology 141, 39–47. https://doi.org/10.1016/j.ygcen.2004. 11.017
Masti, H., Yurnalis, Y., Nova, T.D., Kamsa, Z., Rafian, T., 2021. Keragaman Gen Growth Hormone Receptor (GHR) Ekson 10 pada itik Sikumbang Janti. JIPTHP 9, 15–19. https://doi.org/10.29244/jipthp.9.1.15-19
Noor, R.R., 2010. Genetika Ternak, 6th ed. Penebar Swadaya, Jakarta.
Nova, T.D., Anggraeni, S.D., Wardiansyah, M., Ramadhani, E.P., 2019. Frekuensi Pemberian Ransum Secara Periodik dan Level Protein Terhadap Karkas dan Gambaran Darah Itik Lokal Sikumbang Janti. JPI 21, 64. https://doi.org/10.25077/jpi.21.2.64-75.2019
Nova, T.D., Syahruddin, E., Zein, R., 2020. The Productivity of Duck in Different Temperature Cage Management. J.Nat.Indonesia 18, 43. https://doi.org/10.31258/jnat.18.1.43-61
Purwantini, D., Santosa, R.S.S., Santosa, S.A., Susanto, A., Candrasari, D.P., Ismoyowati, I., 2020. Prolactin gene polymorphisms and associations with reproductive traits in Indonesian local ducks. Vet World 13, 2301–2311. https://doi.org/10.14202/vetworld.2020.2301-2311
Saputro, B.E., Sutrisna, R., Santosa, P.E., Fathul, F., 2016. Pengaruh Ransum yang Berbeda pada Itik Jantan Terhadap Jumlah Leukosit dan Diferensial Leukosit. Jurnal Ilmiah Peternakan Terpadu 4, 176–181.
Setyo Budi, E., Yektiningsih, E., Priyanto, E., 2015. Profitabilitas Usaha Ternak Itik Petelur di Desa Kebonsari Kecamatan Candi, Sidoarjo. AGR 1, 32–37. https://doi.org/10.18196/agr.115
Subekti, K., Solihin, D.D., Afnan, R., Gunawan, A., Sumantri, C., 2019. Polymorphism of Duck HSP70 Gene and mRNA Expression under Heat Stress Conditions. International J. of Poultry Science 18, 591–597. https://doi.org/10.3923/ijps.2019.591.597
Susanti, T., 2015. Prolactin as a Candidate Gene Controlling Molting and Egg Production of Duck. Indonesian Bulletin of Animal and Veterinary Sciences 25, 23–28. https://doi.org/10.14334/wartazoa.v25i1.1125
Susanti, T., Noor, R.R., Hardjosworo, P.S., Prasetyo, L.H., 2012. Relationship Between Prolactin Hormone Level, Molting and Duck Egg Production. J. Indonesian Trop. Anim. Agric. 37, 161–167. https://doi.org/10.14710/jitaa.37.3.161-167
Wang, C., Liang, Z., Liang, Z., Yu, W., Yu, W., Feng, Y., Feng, Y., Peng, X., Peng, X., Gong, Y., Gong, Y., Li, S., Li, S., 2011. Polymorphism of the prolactin gene and its association with egg production traits in native Chinese ducks. SA J. An. Sci. 41, 63–69. https://doi.org/10.4314/sajas.v41i1.66044
Yurnalis, Y., Husmaini, H., Sabrina, S., 2017. Polymorphisms of Growth Hormone Gene Exon 1 and their Associations with Body Weight in Pitalah and Kumbang Janti Ducks. International J. of Poultry Science 16, 203–208. https://doi.org/10.3923/ijps.2017.203.208